In vitro evaluation of platelet concentrates during storage: Platelet counts and markers of platelet destruction

Transfus Apher Sci. 2007 Dec;37(3):261-8. doi: 10.1016/j.transci.2007.02.006. Epub 2007 Nov 26.

Abstract

Background: Differences in platelet counts are observed by use of automated haematology analyzers making interlaboratory comparison difficult.

Materials and methods: Twenty-eight single-donor platelet concentrates (PCs) were collected. Platelet concentration and markers of platelet destruction were investigated during storage for 11/12 days.

Results: Increasing impedance-immunoplatelet ratio was observed during storage, correlating to platelet fragments, large platelets, platelet density and cell-lysis. High variability was observed for optical-immunoplatelet ratio.

Conclusion: Immunoplatelet count or correction factor calculated by impedance-immunoplatelet ratio should be used to confirm that platelet unit meets platelet count requirements or to compare results from clinical trials. Optical platelet count is not recommended.

Publication types

  • Evaluation Study

MeSH terms

  • Blood Platelets / cytology*
  • Electric Impedance
  • Humans
  • Platelet Count / methods*
  • Preservation, Biological*
  • Time Factors