Abstract
The A-ZIP/F-1 transgenic mouse is a model of lipoatrophic diabetes with severe insulin resistance, hyperglycemia and hyperlipidemia. Recently, a regulatory role of adipose tissue on adrenal gland function and blood pressure has been suggested. To further explore the importance of adipose tissue in the regulation of adrenal function and blood pressure, we studied this mouse model of lipodystrophy. A-ZIP/F-1 mice exhibit significantly elevated systolic and diastolic blood pressure values despite lack of white adipose tissue and its hormones. Furthermore, A-ZIP/F-1 lipoatrophic mice have a significant reduction of adrenal zona glomerulosa, while plasma aldosterone levels and aldosterone synthase mRNA expression remain unchanged. On the other hand, lipoatrophic mice present elevated corticosterone levels but no adrenocortical hyperplasia. Ultrastructural analysis of adrenal gland show significant alterations in adrenocortical cells, with conformational changes of mitochondrial internal membranes and high amounts of liposomes. In conclusion, lipodystrophy in A-ZIP/F-1 mice is associated with hypertension, possibly due to hypercorticosteronemia and/or others metabolic-vascular changes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipokines / blood
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Adipose Tissue, White / metabolism*
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Adipose Tissue, White / pathology
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Adrenal Cortex / diagnostic imaging
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Adrenal Cortex / enzymology
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Adrenal Cortex / metabolism*
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Aldosterone / blood
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Animals
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Blood Glucose / metabolism
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Blood Pressure
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Corticosterone / blood
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Cytochrome P-450 CYP11B2 / genetics
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Cytochrome P-450 CYP11B2 / metabolism
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Diabetes Mellitus, Lipoatrophic / complications*
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Diabetes Mellitus, Lipoatrophic / genetics
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Diabetes Mellitus, Lipoatrophic / metabolism
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Diabetes Mellitus, Lipoatrophic / pathology
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Diabetes Mellitus, Lipoatrophic / physiopathology
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Disease Models, Animal
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Hypertension / genetics
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Hypertension / metabolism*
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Hypertension / pathology
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Hypertension / physiopathology
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Insulin / blood
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Lipids / blood
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Male
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Mice
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Mice, Transgenic
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Microscopy, Electron
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Mitochondria / metabolism
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Mitochondria / ultrastructure
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Mitochondrial Membranes / metabolism
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Mitochondrial Membranes / ultrastructure
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RNA, Messenger / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Ultrasonography
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Zona Glomerulosa / metabolism
Substances
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Adipokines
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Blood Glucose
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Insulin
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Lipids
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RNA, Messenger
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Tg(AZIP-F)1Vsn protein, mouse
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Transcription Factors
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Aldosterone
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Cytochrome P-450 CYP11B2
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Corticosterone