Plant pathogenic bacteria transfer effector proteins into plant cells via the hypersensitive response and pathogenicity (Hrp) type III protein secretion system (T3SS) during infection. The genes encoding the Hrp T3SS are expressed only under plant apoplast-mimicking conditions in an AraC-type transcriptional activator HrpB-dependent manner. To identify the proteins controlled by HrpB in Burkholderia glumae in vitro, we constitutively expressed hrpB and analyzed the proteins showing altered expression using 2-DE and ESI-MS/MS. Among 46 proteins exhibiting consistently altered expression, which were encoded by 34 different genes, 34 were secretory proteins and 12 were cytoplasmic. Twenty-eight of the secreted proteins showed increased accumulation, whereas the other six showed decreased accumulation. None of the HrpB-dependent proteins had significant homology to known T3SS-dependent proteins, except for HrpK from Pseudomonas syringae pv. syringae and two T3SS-associated cytoplasmic proteins from Ralstonia solanacearum. Twenty-one of the 34 genes had putative HrpB-binding sequences in their upstream regulatory regions. Secretion of all 34 extracellular proteins was independent of the Hrp T3SS, and 16 were secreted via a type II protein secretion system (T2SS). Mutants lacking the T2SS or Hrp T3SS produced toxoflavin but were less virulent to rice panicles, indicating the importance of these proteins in pathogenicity.