Abstract
We evaluated the one-step multiplex real-time reverse transcription-PCR ProFlu-1 assay for the detection of influenza A and influenza B viruses and respiratory syncytial viruses from 353 pediatric nasopharyngeal aspirates. As assessed by comparison with the results of immunofluorescence testing and cell culture, the specificity and the sensitivity of the ProFlu-1 assay ranged from 97% to 100%. In addition, the ProFlu-1 assay amplified 9% of samples not detected by conventional methods.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Adolescent
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Child
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Child, Preschool
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Fluorescent Antibody Technique / methods
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Humans
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Infant
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Infant, Newborn
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Influenza A virus / genetics
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Influenza A virus / isolation & purification*
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Influenza B virus / genetics
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Influenza B virus / isolation & purification*
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Influenza, Human / diagnosis*
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Pharynx / virology
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Respiratory Syncytial Virus Infections / diagnosis*
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Respiratory Syncytial Viruses / genetics
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Respiratory Syncytial Viruses / isolation & purification*
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Virus Cultivation / methods