Telomere shortening in human HL60 cells by treatment with 3'-azido-2',3'-dideoxynucleosides and telomerase inhibition by their 5'-triphosphates

Xiaohong Liu; Motoko Inomata; Tsukasa Ogawara; Mineo Saneyoshi; Toyofumi Yamaguchi

doi:10.1080/15257770701515468

Telomere shortening in human HL60 cells by treatment with 3'-azido-2',3'-dideoxynucleosides and telomerase inhibition by their 5'-triphosphates

Nucleosides Nucleotides Nucleic Acids. 2007;26(8-9):1067-71. doi: 10.1080/15257770701515468.

Authors

Xiaohong Liu¹, Motoko Inomata, Tsukasa Ogawara, Mineo Saneyoshi, Toyofumi Yamaguchi

Affiliation

¹ Biotechnology Research Center, Teikyo University of Science and Technology, Uenohara, Yamanashi, Japan.

PMID: 18058538
DOI: 10.1080/15257770701515468

Abstract

Telomerase is thought to play an important role in the mechanism of tumor cell immortalization by maintenance of telomere length. To obtain information on the susceptibility of telomerase to nucleoside analogues, the effects of base-modified 3'-azido-2',3'-dideoxynucleoside triphosphates on the enzyme were investigated. It is suggested that the 2-amino group of the nucleotide purine nucleus is important for the inhibitory activity. Telomere shortening caused by long-term treatment with these nucleosides is also described.

MeSH terms

Dideoxyadenosine / analogs & derivatives
Dideoxyadenosine / chemistry
Dideoxyadenosine / pharmacology
Dideoxynucleosides / chemistry
Dideoxynucleosides / pharmacology*
Dideoxynucleotides / chemistry
Dideoxynucleotides / pharmacology*
Drug Design
Enzyme Inhibitors / chemistry
Enzyme Inhibitors / pharmacology
HL-60 Cells
Humans
Telomerase / antagonists & inhibitors*
Telomere / drug effects*

Substances

Dideoxynucleosides
Dideoxynucleotides
Enzyme Inhibitors
3'-azido-2,6-diaminopurine-2',3'-dideoxyriboside
Dideoxyadenosine
Telomerase