Synthesis and biophysical characterization of G-rich oligonucleotides conjugated with sugar-phosphate tails

J D'Onofrio; E Erra; G Di Fabio; A Iadonisi; L Petraccone; L De Napoli; G Barone; J Balzarini; C Giancola; D Montesarchio

doi:10.1080/15257770701528164

Synthesis and biophysical characterization of G-rich oligonucleotides conjugated with sugar-phosphate tails

Nucleosides Nucleotides Nucleic Acids. 2007;26(10-12):1225-9. doi: 10.1080/15257770701528164.

Authors

J D'Onofrio¹, E Erra, G Di Fabio, A Iadonisi, L Petraccone, L De Napoli, G Barone, J Balzarini, C Giancola, D Montesarchio

Affiliation

¹ Dipartimento di Chimica Organica e Biochimica, Università degli Studi di Napoli Federico II, Via Cintia 4, I-80126 Naples, Italy.

PMID: 18066757
DOI: 10.1080/15257770701528164

Abstract

A number of 5'-and 3'-glycoconjugates of the oligonucleotide (5')d(TGGGAG)(3') have been synthesized, exploiting fully automated, online phosphoramidite-based solid phase strategy, as potential anti-HIV-1 agents. The thermodynamic stability of the resulting quadruplexes has been investigated by thermal denaturation studies, via a detailed CD Q1 analysis.

MeSH terms

Anti-HIV Agents / chemical synthesis
Anti-HIV Agents / chemistry*
Glycoconjugates / chemical synthesis
Glycoconjugates / chemistry*
Guanosine / genetics
HIV-1*
Oligodeoxyribonucleotides / chemical synthesis
Oligodeoxyribonucleotides / chemistry*
Sugar Phosphates / chemistry

Substances

Anti-HIV Agents
Glycoconjugates
Oligodeoxyribonucleotides
Sugar Phosphates
Guanosine