Abstract
Porphyrinmaleimides were synthesized and characterized. The thiol-containing amino acid L-cysteine reacted with 58% yield with these porphyrins to form bioconjugate adducts. The new thiol-active reagents were labeled cytoplasmic cysteine 140 and 316 in rhodopsin (Rh), a G protein coupled receptor (GPCR).
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Cysteine / analysis*
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Cysteine / chemistry*
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Electrophoresis, Polyacrylamide Gel
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Light
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Maleimides / chemical synthesis*
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Maleimides / chemistry*
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Porphyrins / chemistry*
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Rhodopsin / chemistry*
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Sulfhydryl Compounds / chemistry*
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Ultraviolet Rays
Substances
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Maleimides
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Porphyrins
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Sulfhydryl Compounds
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maleimide
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Rhodopsin
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Cysteine