Abstract
The activation of Toll-like receptors (TLRs) in dendritic cells (DCs) triggers a rapid inflammatory response to pathogens. However, this response must be tightly regulated because unrestrained TLR signaling generates a chronic inflammatory milieu that often leads to autoimmunity. We have found that the TAM receptor tyrosine kinases-Tyro3, Axl, and Mer-broadly inhibit both TLR and TLR-induced cytokine-receptor cascades. Remarkably, TAM inhibition of inflammation is transduced through an essential stimulator of inflammation-the type I interferon receptor (IFNAR)-and its associated transcription factor STAT1. TLR induction of IFNAR-STAT1 signaling upregulates the TAM system, which in turn usurps the IFNAR-STAT1 cassette to induce the cytokine and TLR suppressors SOCS1 and SOCS3. These results illuminate a self-regulating cycle of inflammation, in which the obligatory, cytokine-dependent activation of TAM signaling hijacks a proinflammatory pathway to provide an intrinsic feedback inhibitor of both TLR- and cytokine-driven immune responses.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Axl Receptor Tyrosine Kinase
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Dendritic Cells / metabolism*
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Gene Expression Regulation
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Immunity, Innate*
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Inflammation / prevention & control*
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Mice
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Mice, Knockout
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Oncogene Proteins / physiology
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Proto-Oncogene Proteins / physiology
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Receptor Protein-Tyrosine Kinases / metabolism*
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Receptor Protein-Tyrosine Kinases / physiology
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Receptor, Interferon alpha-beta / metabolism
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STAT1 Transcription Factor / metabolism
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Signal Transduction
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Suppressor of Cytokine Signaling 1 Protein
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Suppressor of Cytokine Signaling 3 Protein
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Suppressor of Cytokine Signaling Proteins / metabolism
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Toll-Like Receptors / physiology*
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Ubiquitination
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c-Mer Tyrosine Kinase
Substances
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Ifnar1 protein, mouse
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Oncogene Proteins
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Proto-Oncogene Proteins
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STAT1 Transcription Factor
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Socs1 protein, mouse
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Socs3 protein, mouse
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Stat1 protein, mouse
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Suppressor of Cytokine Signaling 1 Protein
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Suppressor of Cytokine Signaling 3 Protein
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Suppressor of Cytokine Signaling Proteins
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Toll-Like Receptors
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Receptor, Interferon alpha-beta
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Mertk protein, mouse
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Receptor Protein-Tyrosine Kinases
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Tyro3 protein, mouse
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c-Mer Tyrosine Kinase
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Axl Receptor Tyrosine Kinase
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AXL receptor tyrosine kinase, mouse