Abstract
To investigate the maintenance of long-term memory B cells specific for hepatitis B surface antigen (HBsAg), purified blood B cells were polyclonally stimulated and cells secreting antibodies directed to HBsAg (HBs-SC) enumerated by ELISpot. HBs-SC were found in 18/20 HIV-1-infected patients with either natural or vaccinal immunity to hepatitis B virus, including six subjects with serum anti-hepatitis B surface antibody levels less than 10 mIU/ml. A lower number of HBs-SC was found in HBsAg-vaccinated patients compared with vaccinated controls.
MeSH terms
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Anti-HIV Agents / therapeutic use
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Antibodies, Viral / analysis
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B-Lymphocytes / immunology*
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B-Lymphocytes / virology
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Case-Control Studies
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Enzyme-Linked Immunosorbent Assay
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HIV Infections / drug therapy
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HIV Infections / immunology*
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HIV Infections / virology
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HIV-1*
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Hepatitis B Surface Antigens / blood*
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Hepatitis B Surface Antigens / immunology
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Hepatitis B Vaccines / administration & dosage
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Hepatitis B virus / immunology*
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Hepatitis B, Chronic / immunology*
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Hepatitis B, Chronic / prevention & control
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Hepatitis B, Chronic / virology
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Humans
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Immunologic Memory
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Lymphocyte Activation
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Lymphocyte Count
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Statistics, Nonparametric
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Time Factors
Substances
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Anti-HIV Agents
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Antibodies, Viral
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Hepatitis B Surface Antigens
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Hepatitis B Vaccines