Abstract
The interferon inducible transmembrane (IFITM) proteins mediate several cellular processes such as homotypic cell adhesion functions of interferons (IFNs) and cellular anti-proliferative activities. We show that the BAF complex-mediated induction of IFITM3 is dependent on binding of the transcriptional enhancer factor 1 (TEF-1/TEAD1) to the M-CAT like elements of its promoter. TEF-1 knock-down reduced the BAF complex-mediated activation of IFITM3 promoter. In the absence of the BAF complex, TEF-1 is repressive to IFITM3 expression. The regulation of IFITM3 by TEF-1 demonstrates that TEF-1 dependent regulation is more widespread than its previously established role in the expression of muscle specific genes.
Publication types
-
Research Support, N.I.H., Intramural
MeSH terms
-
Amino Acid Sequence
-
Base Sequence
-
Cell Line
-
DNA Helicases / physiology*
-
DNA-Binding Proteins / physiology*
-
Electrophoretic Mobility Shift Assay
-
Gene Expression Regulation / physiology*
-
Humans
-
Membrane Proteins / chemistry
-
Membrane Proteins / genetics*
-
Molecular Sequence Data
-
Nuclear Proteins / physiology*
-
Promoter Regions, Genetic
-
RNA Interference
-
RNA-Binding Proteins / chemistry
-
RNA-Binding Proteins / genetics*
-
Sequence Homology, Nucleic Acid
-
TEA Domain Transcription Factors
-
Transcription Factors / physiology*
Substances
-
DNA-Binding Proteins
-
IFITM3 protein, human
-
Membrane Proteins
-
Nuclear Proteins
-
RNA-Binding Proteins
-
TEA Domain Transcription Factors
-
TEAD1 protein, human
-
Transcription Factors
-
SMARCA4 protein, human
-
DNA Helicases