CRT1, an Arabidopsis ATPase that interacts with diverse resistance proteins and modulates disease resistance to turnip crinkle virus

Hong-Gu Kang; Joseph C Kuhl; Pradeep Kachroo; Daniel F Klessig

doi:10.1016/j.chom.2007.11.006

CRT1, an Arabidopsis ATPase that interacts with diverse resistance proteins and modulates disease resistance to turnip crinkle virus

Cell Host Microbe. 2008 Jan 17;3(1):48-57. doi: 10.1016/j.chom.2007.11.006.

Authors

Hong-Gu Kang¹, Joseph C Kuhl, Pradeep Kachroo, Daniel F Klessig

Affiliation

¹ Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA.

PMID: 18191794
DOI: 10.1016/j.chom.2007.11.006

Abstract

Plant immunity frequently involves the recognition of pathogen-encoded avirulence (avr) factors by their corresponding plant resistance (R) proteins. This triggers the hypersensitive response (HR) where necrotic lesions formed at the site(s) of infection help restrict pathogen spread. HRT is an Arabidopsis R protein required for resistance to turnip crinkle virus (TCV). In a genetic screen for mutants compromised in the recognition of TCV's avr factor, we identified crt1 (compromised recognition of TCV), a mutant that prematurely terminates an ATPase protein. Following TCV infection, crt1 developed a spreading HR and failed to control viral replication and spread. crt1 also suppressed HR-like cell death induced by ssi4, a constitutively active R protein, and by Pseudomonas syringae carrying avrRpt2. Furthermore, CRT1 interacts with HRT, SSI4, and two other R proteins, RPS2 and Rx. These data identify CRT1 as an important mediator of defense signaling triggered by distinct classes of R proteins.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Adenosine Triphosphatases / chemistry
Adenosine Triphosphatases / genetics
Adenosine Triphosphatases / metabolism*
Amino Acid Sequence
Arabidopsis / genetics
Arabidopsis / microbiology
Arabidopsis / virology*
Arabidopsis Proteins / chemistry
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Base Sequence
Carmovirus / pathogenicity*
Consensus Sequence
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Endodeoxyribonucleases / chemistry
Endodeoxyribonucleases / genetics
Endodeoxyribonucleases / metabolism*
Gene Expression Regulation, Plant*
Immunity, Innate*
Molecular Sequence Data
Mutation
Nicotiana / virology
Plant Diseases / virology
Plant Viruses / pathogenicity
Repressor Proteins / genetics
Repressor Proteins / metabolism

Substances

Arabidopsis Proteins
DNA-Binding Proteins
HRT protein, Arabidopsis
Repressor Proteins
CRT1 protein, Arabidopsis
Endodeoxyribonucleases
Adenosine Triphosphatases