A new advanced glycation inhibitor, LR-90, prevents experimental diabetic retinopathy in rats

Br J Ophthalmol. 2008 Apr;92(4):545-7. doi: 10.1136/bjo.2007.127910. Epub 2008 Jan 22.

Abstract

Background: Diabetic retinopathy is associated with accumulation of advanced glycation end products in the retinal microvasculature. LR-90 is an effective multistage inhibitor of advanced glycation with renoprotective and anti-inflammatory properties.

Aim: To explore the role of LR-90 in the progression of experimental diabetic retinopathy.

Methods: Streptozotocin-induced diabetic Sprague-Dawley rats were treated with LR-90 (50 mg/l in drinking water) for up to 32 weeks. At the end of the study, eyes were enucleated and subjected to trypsin digestion and staining with light green/haematoxylin. Acellular capillaries and pericytes were quantified in random fields using light microscopy.

Results: In the LR-90-treated diabetic animals, acellular capillary numbers were reduced to 1.63 (0.20) from 2.58 (0.49) (p<0.05) in diabetic controls. LR-90 treatment also restored the pericyte deficit from 18.12 (0.98) in diabetic rats to 24.19 (0.76) (p<0.001).

Conclusion: These findings show that LR-90 can effectively inhibit important lesions of diabetic retinopathy. This agent has potential for preventing retinopathy in patients with diabetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Weight / drug effects
  • Butyrates / therapeutic use*
  • Cholesterol / blood
  • Creatinine / urine
  • Diabetes Mellitus, Experimental / blood
  • Diabetes Mellitus, Experimental / drug therapy
  • Diabetes Mellitus, Experimental / pathology
  • Diabetic Retinopathy / pathology
  • Diabetic Retinopathy / prevention & control*
  • Disease Progression
  • Drug Evaluation, Preclinical / methods
  • Glycation End Products, Advanced / antagonists & inhibitors*
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Triglycerides / blood

Substances

  • Butyrates
  • Glycation End Products, Advanced
  • LR-90
  • Triglycerides
  • Cholesterol
  • Creatinine