Abstract
The effect of hyperosmotic condition on the outer membrane protein (Omp) composition of Pseudomonas fluorescens was investigated by proteomic analyses. The abundances of 12 proteins, including porins, lipoproteins, and the flagella subunit FliC, were modified. This was at least partly explained by altered gene expression, as shown by mRNA level study. In agreement with Omp changes, hyperosmotic condition resulted in vesicle formation and modifications of mobility and antibiotic susceptibility.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Bacterial Outer Membrane Proteins / metabolism*
-
Electrophoresis, Gel, Two-Dimensional
-
Microbial Sensitivity Tests
-
Microscopy, Electron, Scanning
-
Osmolar Concentration
-
Pseudomonas fluorescens / drug effects
-
Pseudomonas fluorescens / genetics
-
Pseudomonas fluorescens / metabolism*
-
RNA, Messenger / genetics
-
Reverse Transcriptase Polymerase Chain Reaction
-
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
-
Bacterial Outer Membrane Proteins
-
RNA, Messenger