Abstract
S-adenosylmethionine is a metabolite regulating many biological processes; S-adenosylmethionine effect on ubiquitin-proteasome system (UPS) has not been studied yet. We investigated S-adenosylmethionine effects on UPS activity both in vitro, by inhibitor screening assay, and in rat vascular smooth muscle cells, by Western Blot of proteasomal targets. We found that S-adenosylmethionine inhibited UPS activity.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Blood Vessels / drug effects
-
Blood Vessels / enzymology
-
Cells, Cultured
-
Muscle, Smooth, Vascular / drug effects
-
Muscle, Smooth, Vascular / enzymology*
-
Myocytes, Smooth Muscle / drug effects
-
Myocytes, Smooth Muscle / enzymology*
-
Proteasome Endopeptidase Complex / metabolism
-
Proteasome Inhibitors*
-
Rats
-
S-Adenosylmethionine / pharmacology*
-
Ubiquitin / antagonists & inhibitors*
-
Ubiquitin / metabolism
Substances
-
Proteasome Inhibitors
-
Ubiquitin
-
S-Adenosylmethionine
-
Proteasome Endopeptidase Complex