Ypt32p and Mlc1p bind within the vesicle binding region of the class V myosin Myo2p globular tail domain

Mol Microbiol. 2008 Mar;67(5):1051-66. doi: 10.1111/j.1365-2958.2008.06106.x. Epub 2008 Jan 23.

Abstract

Myosin V is an actin-based motor essential for a variety of cellular processes including skin pigmentation, cell separation and synaptic transmission. Myosin V transports organelles, vesicles and mRNA by binding, directly or indirectly, to cargo-bound receptors via its C-terminal globular tail domain (GTD). We have used the budding yeast myosin V Myo2p to shed light on the mechanism of how Myo2p interacts with post-Golgi carriers. We show that the Rab/Ypt protein Ypt32p, which associates with membranes of the trans-Golgi network, secretory vesicles and endosomes and is related to the mammalian Rab11, interacts with the Myo2p GTD within a region previously identified as the 'vesicle binding region'. Furthermore, we show that the essential myosin light chain 1 (Mlc1p), required for vesicle delivery at the mother-bud neck during cytokinesis, binds to the Myo2p GTD in a region overlapping that of Ypt32p. Our data are consistent with a role of Ypt32p and Mlc1p in regulating the interaction of post-Golgi carriers with Myo2p subdomain II.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Amino Acid Substitution
  • Conserved Sequence
  • Gene Expression Regulation, Fungal
  • Molecular Sequence Data
  • Molecular Structure
  • Myosin Heavy Chains / chemistry
  • Myosin Heavy Chains / genetics
  • Myosin Heavy Chains / metabolism*
  • Myosin Light Chains / metabolism
  • Myosin Type V / chemistry
  • Myosin Type V / genetics
  • Myosin Type V / metabolism*
  • Protein Binding
  • Protein Structure, Tertiary
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / chemistry
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Secretory Vesicles / physiology*
  • Secretory Vesicles / ultrastructure
  • Sequence Alignment
  • Two-Hybrid System Techniques
  • rab GTP-Binding Proteins / genetics
  • rab GTP-Binding Proteins / metabolism*

Substances

  • MLC1 protein, S cerevisiae
  • MYO2 protein, S cerevisiae
  • Myosin Light Chains
  • Saccharomyces cerevisiae Proteins
  • Myosin Type V
  • YPT32 protein, S cerevisiae
  • Myosin Heavy Chains
  • rab GTP-Binding Proteins