Spectrophotometric determination of glutathione and of its oxidation product in pharmaceutical dosage forms

M A Raggi; L Nobile; A G Giovannini

doi:10.1016/0731-7085(91)80041-7

Spectrophotometric determination of glutathione and of its oxidation product in pharmaceutical dosage forms

J Pharm Biomed Anal. 1991;9(10-12):1037-40. doi: 10.1016/0731-7085(91)80041-7.

Authors

M A Raggi¹, L Nobile, A G Giovannini

Affiliation

¹ Department of Pharmaceutical Sciences, University of Bologna, Italy.

PMID: 1822170
DOI: 10.1016/0731-7085(91)80041-7

Abstract

A simple and sensitive spectrophotometric method suitable for the stability control of pharmaceutical dosage forms containing glutathione (gamma-glutamyl-cysteinyl-glycine), GSH, is described. Besides GSH, the method quantitatively determines its oxidation product, GSSG. The colour reactions of GSH and GSSG with ammonium tetrachloropalladate have been investigated and the optimum reaction conditions, spectral characteristics and composition of the yellow water-soluble complexes have been established. The assay results of pharmaceutical formulations showed good accuracy and precision over the concentration range of 5 x 10(-5)-6 x 10(-4) M GSH.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Calibration
Dosage Forms
Glutathione / analogs & derivatives*
Glutathione / analysis*
Glutathione Disulfide
Molecular Sequence Data
Sensitivity and Specificity
Spectrophotometry
Spectrophotometry, Ultraviolet

Substances

Dosage Forms
Glutathione
Glutathione Disulfide