Abstract
Rat adipocytes were treated with antisense dimethoxytrityl pentadecadeoxynucleotides, complementary to mRNA initiation codon regions for alpha and beta isozymes of protein kinase C (PKC). This antisense treatment provoked 50-70% decreases in PKC and insulin-stimulated 2-deoxyglucose uptake, but did not inhibit insulin-stimulated diacylglycerol synthesis. Sense or nonsense oligodeoxynucleotides were without effect on PKC and 2-deoxyglucose uptake. These results suggest that: (i) PKC-alpha and PKC-beta isozymes can be specifically downregulated in rat adipocytes by antisense oligodeoxynucleotides, and (ii) insulin-stimulated glucose transport requires PKC.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adipose Tissue / cytology
-
Adipose Tissue / drug effects*
-
Adipose Tissue / metabolism
-
Animals
-
Base Sequence
-
Biological Transport / drug effects
-
Cells, Cultured
-
DNA, Antisense / genetics
-
DNA, Antisense / pharmacology*
-
Deoxyglucose / metabolism*
-
Down-Regulation / drug effects
-
Down-Regulation / genetics
-
Glucose / metabolism
-
Insulin Antagonists / pharmacology
-
Isoenzymes / metabolism*
-
Male
-
Molecular Sequence Data
-
Protein Kinase C / metabolism*
-
Rats
-
Rats, Inbred Strains
Substances
-
DNA, Antisense
-
Insulin Antagonists
-
Isoenzymes
-
Deoxyglucose
-
Protein Kinase C
-
Glucose