Using coupled monolithic rods for ultra-high peak capacity LC and LC-MS under normal LC operating pressures

Analyst. 2008 Feb;133(2):180-3. doi: 10.1039/b714807k. Epub 2007 Dec 17.

Abstract

Up to ten individual reversed-phase monolithic LC columns were efficiently coupled to equal a total column length of 1 m. The column generated up to 90,000 theoretical plates under optimum conditions with a peak capacity under gradient conditions of 244 peaks h(-1) at a total column backpressure of just 140 bar, directly coupled to ESI-MS.

MeSH terms

  • Animals
  • Chromatography, Liquid / instrumentation*
  • Chromatography, Liquid / methods
  • Humans
  • Proteins / analysis
  • Sensitivity and Specificity
  • Spectrometry, Mass, Electrospray Ionization / methods

Substances

  • Proteins