Abstract
Selective immunoprecipitation of proteins is a useful tool for characterizing proteins and protein-protein interactions. Clear step-by-step protocols are provided for preparing lysates of cells and yeast under a variety of conditions, for binding the antibody to a solid matrix, and for performing the actual immunoprecipitation. An additional method is provided for increasing the specificity of the technique by reprecipitating the antigen with the same or a different antibody.
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology
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Antigen-Antibody Reactions
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Cells, Cultured / chemistry
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Detergents
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Fungal Proteins / immunology
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Fungal Proteins / isolation & purification
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Humans
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Immunoprecipitation / instrumentation
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Immunoprecipitation / methods*
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Isotope Labeling / methods
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Proteins / immunology
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Proteins / isolation & purification*
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Radioimmunoprecipitation Assay / methods
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Radioisotopes / analysis
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Saccharomyces cerevisiae / chemistry
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Specimen Handling
Substances
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Antibodies, Monoclonal
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Detergents
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Fungal Proteins
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Proteins
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Radioisotopes