We describe a new species of Chilicola (Hylaeosoma) of the megalostigma group from southern Colombia and present an identification key to species. Chilicola muruimuinane sp. nov. resembles Chilicola yanezae Hinojosa-Díaz & Michener, 2005, from Mexico, but it differs from it in its body color, shape of basal tarsomeres of female fore legs, and characters of the seventh and eighth sterna of the male.