Abstract
A recombinant hst-1 protein produced in silkworm cells by a recombinant baculovirus, previously shown to be a potent mitogen for NIH3T3 cells and human endothelial cells, also stimulated anchorage-independent growth of NRK-49F cells. Amino acid sequence analysis revealed that the amino-terminal sequence with 58 amino acids was cleaved off in silkworm cells. These results indicated that the mature hst-1 protein consisting of 148 amino acids had transforming growth factor activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Baculoviridae / metabolism
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Bombyx / metabolism
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Cell Division / drug effects
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Cells, Cultured
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Endothelium / drug effects
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Fibroblast Growth Factor 4
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Fibroblast Growth Factors / chemistry*
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Fibroblast Growth Factors / pharmacology
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Growth Substances / chemistry*
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Growth Substances / pharmacology
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Humans
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Molecular Sequence Data
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Oncogene Proteins / chemistry*
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Oncogene Proteins / pharmacology
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Proto-Oncogene Proteins / chemistry*
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Proto-Oncogene Proteins / pharmacology
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Transforming Growth Factors / chemistry
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Transforming Growth Factors / metabolism*
Substances
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FGF4 protein, human
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Fibroblast Growth Factor 4
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Growth Substances
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Oncogene Proteins
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Proto-Oncogene Proteins
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Fibroblast Growth Factors
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Transforming Growth Factors