[Combined assay of soluble CD30 and hepatocyte growth factor for diagnosis of acute renal allograft rejection]

Nan Fang Yi Ke Da Xue Xue Bao. 2008 Feb;28(2):241-2.
[Article in Chinese]

Abstract

Objective: To study the value of detection of both preoperative soluble CD30 (sCD30) and hepatocyte growth factor (HGF) level 5 days after transplantation in the diagnosis of acute rejection of renal allograft.

Methods: Preoperative serum sCD30 levels and HGF level 5 days after transplantation were determined in 65 renal-transplant recipients using enzyme-linked immunosorbent assay. The recipients were divided according to the sCD30 levels positivity. Receiver operating characteristic (ROC) curves were used to assess the value of HGF level on day 5 posttransplantation for diagnosis of acute renal allograft rejection, and the value of combined assay of the sCD30 and HGF levels was also estimated.

Results: After transplantation, 26 recipients developed graft rejection and 39 had uneventful recovery without rejection. With the cut-off value of sCD30 of 120 U/ml, the positivity rate of sCD30 was significantly higher in recipients with graft rejection than in those without (61.5% vs 17.9%, P<0.05). Recipients with acute rejection showed also significantly higher HGF levels on day 5 posttransplantation than those without rejection (P<0.05). ROC curve analysis indicated that HGF levels on day 5 posttransplantation was a good marker for diagnosis of acute renal allograft rejection, and at the cut-off value of 90 ug/L, the diagnostic sensitivity was 84.6% and specificity 76.9%. Evaluation of both the sCD30 and HGF levels significantly enhanced the diagnostic accuracy of acute graft rejection.

Conclusion: Combined assay of serum sCD30 and HGF levels offers a useful means for diagnosis of acute renal allograft rejection.

MeSH terms

  • Enzyme-Linked Immunosorbent Assay
  • Graft Rejection / blood
  • Graft Rejection / diagnosis*
  • Hepatocyte Growth Factor / blood*
  • Humans
  • Ki-1 Antigen / blood*
  • Kidney Transplantation*
  • ROC Curve
  • Sensitivity and Specificity
  • Transplantation, Homologous

Substances

  • Ki-1 Antigen
  • Hepatocyte Growth Factor