Open-source platform for the analysis of liquid chromatography-mass spectrometry (LC-MS) data

Matthew Fitzgibbon; Wendy Law; Damon May; Andrea Detter; Martin McIntosh

doi:10.1007/978-1-59745-117-8_19

Open-source platform for the analysis of liquid chromatography-mass spectrometry (LC-MS) data

Methods Mol Biol. 2008:428:369-82. doi: 10.1007/978-1-59745-117-8_19.

Authors

Matthew Fitzgibbon¹, Wendy Law, Damon May, Andrea Detter, Martin McIntosh

Affiliation

¹ Fred Hutchinson Cancer Research Center, Seattle, WA, USA.

PMID: 18287783
DOI: 10.1007/978-1-59745-117-8_19

Abstract

The analysis of protein mixtures by liquid chromatography-mass spectrometry (LCMS) requires tools for viewing and navigating LC-MS data, locating peptides in LC-MS data, and eliminating low-quality peptides. msInspect, an open source platform, can carry out these steps for single experiments and can align and normalize peptide features in comparative studies with multiple LC-MS runs. In addition, msInspect can analyze quantitative studies with and without isotopic labels to generate peptide arrays.

MeSH terms

Algorithms
Chromatography, Liquid / statistics & numerical data*
Data Interpretation, Statistical
Humans
Mass Spectrometry / statistics & numerical data*
Peptides / analysis
Protein Array Analysis / statistics & numerical data
Proteome / analysis
Proteomics / statistics & numerical data*
Software*

Substances

Peptides
Proteome