Abstract
We cloned a plant gene, Ntcyc07, conferring arsenite tolerance by expressing a tobacco expression library in WT yeast (Y800). Expression of Ntcyc07 increased the tolerance to As(III) and decreased its accumulation, suggesting that the enhanced As(III) tolerance resulted from a reduction of the intracellular arsenic level. Interestingly, expression of Ntcyc07 increased the expression of the As(III) export carrier ACR3, but repressed that of As(III) uptake channel FPS1. Ntcyc07p interacted with Acr1p, which is the transcriptional activator of ACR3, but not with the ACR3 promoter. Taken together, the data indicated that Ntcyc07p promoted As(III) tolerance by decreasing the intracellular level of As(III) via increasing the expression of ACR3 and reducing that of FPS1.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Arsenic / analysis
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Arsenic / metabolism*
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Arsenites / pharmacology*
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Basic-Leucine Zipper Transcription Factors / metabolism
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Cloning, Molecular
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Drug Resistance / genetics*
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Genes, Plant*
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Membrane Proteins / antagonists & inhibitors
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Membrane Transport Proteins / genetics
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Membrane Transport Proteins / metabolism
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Molecular Sequence Data
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Nicotiana / drug effects*
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Nicotiana / genetics*
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Nicotiana / metabolism
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Repressor Proteins / metabolism
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / antagonists & inhibitors
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
Substances
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ACR3 protein, S cerevisiae
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Arsenites
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Basic-Leucine Zipper Transcription Factors
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FPS1 protein, S cerevisiae
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Membrane Proteins
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Membrane Transport Proteins
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Repressor Proteins
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SKO1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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arsenite
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Arsenic