Serum cystatin C in the United States: the Third National Health and Nutrition Examination Survey (NHANES III)

Am J Kidney Dis. 2008 Mar;51(3):385-94. doi: 10.1053/j.ajkd.2007.11.019.

Abstract

Background: Serum cystatin C increasingly is used as a marker of glomerular filtration rate and cardiovascular risk. However, information for serum cystatin C levels in the general population, specifically across a wide age range and different ethnicities, is lacking.

Objectives: To determine nationally representative serum cystatin C levels, estimate the prevalence of increased cystatin C levels in the general population, and identify factors associated with increased cystatin C levels.

Study design: Cross-sectional survey.

Setting and participants: A nationally representative subsample of 7,596 participants aged 12 years or older in the Third National Health and Nutrition Examination Survey conducted in 1988-1994.

Predictors: Age, sex, race/ethnicity, risk factors for chronic kidney disease.

Outcomes: Continuous serum cystatin C levels and serum cystatin C level greater than 1.12 mg/L.

Measurements: Cystatin C was measured in 2006 from stored sera by using an automated particle-enhanced nephelometric assay.

Results: Overall median serum cystatin C level was 0.85 mg/L. Median cystatin C levels increased steeply with age and were greater in males and non-Hispanic white persons, even in a healthy subgroup of 20- to 39-year-olds. Prevalences of increased serum cystatin C levels (>1.12 mg/L) were 1%, 41%, and greater than 50% in all persons aged younger than 20 years, 60 years or older, and 80 years or older. In persons aged 60 years and older, older age, non-Hispanic white ethnicity, hypertension, current smoking, lower levels of education and high-density lipoprotein cholesterol, and increased body mass index, C-reactive protein, and triglyceride values were associated significantly with increased serum cystatin C levels.

Limitations: No measured glomerular filtration rate, single measurement of cystatin C, cross-sectional study design.

Conclusions: Serum cystatin C level is related to sex and ethnicity, even in young healthy individuals. The prevalence of increased cystatin C levels increases dramatically with age, reaching greater than 50% after the age of 80 years in both sexes and all ethnic groups.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Age Factors
  • Aged
  • Biomarkers / blood*
  • Cross-Sectional Studies
  • Cystatin C
  • Cystatins / blood*
  • Diabetes Mellitus / blood
  • Female
  • Glomerular Filtration Rate
  • Humans
  • Hypertension / blood
  • Kidney Function Tests / methods*
  • Logistic Models
  • Male
  • Middle Aged
  • Nephelometry and Turbidimetry
  • Nutrition Surveys
  • Reference Values
  • United States

Substances

  • Biomarkers
  • CST3 protein, human
  • Cystatin C
  • Cystatins