Olfactory ensheathing cells conditioned medium prevented apoptosis induced by 6-OHDA in PC12 cells through modulation of intrinsic apoptotic pathways

Lei Feng; Haiwei Meng; Fengxia Wu; Baohua Cheng; Xiuquan He; Xiaohong Wang; Zhengping Li; Shuwei Liu

doi:10.1016/j.ijdevneu.2008.01.003

Olfactory ensheathing cells conditioned medium prevented apoptosis induced by 6-OHDA in PC12 cells through modulation of intrinsic apoptotic pathways

Int J Dev Neurosci. 2008 May-Jun;26(3-4):323-9. doi: 10.1016/j.ijdevneu.2008.01.003. Epub 2008 Jan 18.

Authors

Lei Feng¹, Haiwei Meng, Fengxia Wu, Baohua Cheng, Xiuquan He, Xiaohong Wang, Zhengping Li, Shuwei Liu

Affiliation

¹ Department of Anatomy, Shandong University School of Medicine, Jinan, Shandong, PR China.

PMID: 18295431
DOI: 10.1016/j.ijdevneu.2008.01.003

Abstract

Olfactory ensheathing cells (OECs) express a high level of growth factors which play a very important role as neuronal support. Recent evidence in literatures showed that transplantation of OECs may improve functional restoration in 6-OHDA-induced rat model of Parkinson's disease (PD). However, the biological function of various factors released from OECs in Parkinson' disease is still unclear. In this study, we examined the effects of newborn rat OECs conditioned medium (CM) on PC12 cells. Cells treated with 6-OHDA underwent cytotoxicity and apoptotic death determined by MTT assay and Hoechst 33342/PI staining. OECs CM was able to reduce the cellular damage in PC12 cells. Further investigation results showed that CM inhibited the disruption of mitochondrial transmembrane potential, up-regulation of Bcl-2 and down-regulation of Bax. Taken together, this study indicates that CM has a neuroprotective effect on 6-OHDA induced apoptosis of PC12 cells, which is through up-regulation of the Bcl-2/Bax ratio and protection for mitochondrion.

MeSH terms

Animals
Animals, Newborn
Apoptosis / drug effects*
Apoptosis / physiology
Benzimidazoles
Biological Assay
Cells, Cultured
Culture Media, Conditioned / metabolism
Culture Media, Conditioned / pharmacology*
Indicators and Reagents
Membrane Potential, Mitochondrial / drug effects
Membrane Potential, Mitochondrial / physiology
Nerve Degeneration / chemically induced
Nerve Degeneration / metabolism
Nerve Degeneration / prevention & control*
Nerve Growth Factors / metabolism
Nerve Growth Factors / pharmacology*
Neuroglia / cytology
Neuroglia / metabolism*
Neurons / drug effects
Neurons / metabolism
Neurotoxins
Olfactory Bulb / cytology
Olfactory Bulb / metabolism*
Oxidopamine
PC12 Cells
Proto-Oncogene Proteins c-bcl-2 / drug effects
Proto-Oncogene Proteins c-bcl-2 / metabolism
Rats
Tetrazolium Salts
Thiazoles
bcl-2-Associated X Protein / drug effects
bcl-2-Associated X Protein / metabolism

Substances

Benzimidazoles
Culture Media, Conditioned
Indicators and Reagents
Nerve Growth Factors
Neurotoxins
Proto-Oncogene Proteins c-bcl-2
Tetrazolium Salts
Thiazoles
bcl-2-Associated X Protein
Oxidopamine
thiazolyl blue
bisbenzimide ethoxide trihydrochloride