Objective: To study refinement of macroporous resin to Huang-Lian-Jie-Du decoction.
Methods: The content changes of berberine, geniposide and total alkaloid in the solution which went through the macroporous resin were determined by TLC or spectrophotometry. The type of resin, absorption and desorption condition were optimized.
Results: YWD-09D macroporous resin was suitable for refinement of Huang-Lian-Jie-Du decoction. The absorptive capacity of the resin was 1: 1.3. The concentration of solution was 0.4 g/ml, pH 4 and current velocity was 2 ml/min. The ratio of diameter and high of the resin column was 1:20. The desorption solution was 90% alcohol of 4BV.
Conclusion: YWD-09D macroporous resin and its selected condition are suitable for refinement of Huang-Lian-Jie-Du decoction.