Effects of alcohol consumption on the allergen-specific immune response in mice

Alcohol Clin Exp Res. 2008 Mar;32(3):553-6. doi: 10.1111/j.1530-0277.2008.00644.x.

Abstract

Background: There is evidence that chronic alcohol consumption impairs the T-helper 1 (Th1) lymphocyte-regulated cell-mediated immune response possibly favoring a Th2 deviation of the immune response. Moreover, a few epidemiological studies have linked alcohol consumption to allergen-specific IgE sensitization.

Objective: To investigate the effects of alcohol consumption on the allergen-specific immune response in mice.

Methods: BALB/cJBomTac mice were immunized intraperitoneally with ovalbumin (OVA) using a low dose sensitization protocol. Throughout the experiment, mice were kept on isocalorical liquid diets containing 0 to 6.2% ethanol. Evaluation of immunomodulatory effects of ethanol was based on measurements of total serum IgE, as well as OVA-specific IgE, IgG1, and IgG2a. Furthermore, levels of OVA-induced interleukin (IL)-4 and interferon-gamma were determined in ex vivo splenocyte cultures.

Results: Alcohol intake decreased the level of OVA-specific IgG2a in a dose-dependent manner, whereas high levels of alcohol markedly increased the level of total IgE, but not OVA-specific IgE. Th1 suppression was supported by the cytokine profile.

Conclusions: Alcohol consumption induced a marked decrease in markers of the Th1-type allergen-specific immune response and an increase in total serum IgE. In this model, there was no effect of alcohol on OVA-specific IgE. Studies using other routes of immunization may be warranted.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Drinking / blood*
  • Alcohol Drinking / immunology*
  • Allergens / immunology*
  • Animals
  • Cells, Cultured
  • Ethanol / administration & dosage
  • Female
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin G / blood
  • Mice
  • Mice, Inbred BALB C
  • Spleen / drug effects
  • Spleen / immunology
  • Spleen / metabolism

Substances

  • Allergens
  • Immunoglobulin G
  • Ethanol