The addition of a new immunomodulator with the adjuvant adaptation ADAD system using fatty acid binding proteins increases the protection against Fasciola hepatica

Vet Parasitol. 2008 May 6;153(1-2):176-81. doi: 10.1016/j.vetpar.2008.01.023. Epub 2008 Jan 20.

Abstract

Fatty acid binding proteins (FABP) have shown protective immune response against Fasciola hepatica infection. We evaluated the protection induced by the Fh12 FABP from F. hepatica (Fh12) combined with the new immunomodulator the lipidic aminoalcohol OA0012 in the ADAD system in mice and sheep. In this work we introduced a lipidic aminoalcohol OA0012 as immunomodulator alone or in combination with the hydroalcoholic extract of Phlebodium pseudoaureum; PAL. Mice vaccinated with ADAD containing OA0012+Fh12 or OA0012+Qs+Fh12 had survival rates of 40-50%. Sheep ADAD-vaccinated with OA0012+Qs+Fh12 showed lower fluke recovery, less hepatic lesions and higher post-infection daily weight gain than F. hepatica infected control animals. Sheep ADAD-vaccinated with OA0012 combined PAL and Qs+Fh12 showed lower fluke recovery (42%), lower adult worms count (57%) lower faecal egg count (38%), less hepatic lesions and higher post-infection daily weight gain than F. hepatica infected control animals. Thus, the addition of a new immunomodulator of synthesis to ADAD system with FABPs increased the protection against F. hepatica.

Publication types

  • Controlled Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / administration & dosage*
  • Animals
  • Antibodies, Helminth / blood
  • Antibodies, Helminth / immunology
  • Fasciola hepatica / immunology*
  • Fascioliasis / prevention & control
  • Fascioliasis / veterinary*
  • Fatty Acid-Binding Proteins / administration & dosage*
  • Female
  • Immunoglobulin G / blood
  • Immunologic Factors / administration & dosage*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Sheep
  • Sheep Diseases / immunology
  • Sheep Diseases / parasitology
  • Sheep Diseases / prevention & control
  • Vaccines / immunology*

Substances

  • Adjuvants, Immunologic
  • Antibodies, Helminth
  • Fatty Acid-Binding Proteins
  • Immunoglobulin G
  • Immunologic Factors
  • Vaccines