A novel anti-inflammatory peptide from human lipocortin 5

Br J Pharmacol. 1991 Jun;103(2):1327-32. doi: 10.1111/j.1476-5381.1991.tb09788.x.

Abstract

1. A novel anti-inflammatory peptide (residues 204-212) of human recombinant lipocortin 5 (hrLC5) found on the high similarity region with uteroglobin is described. 2. Peptide 204-212 dose-dependently inhibited the contractions of rat isolated stomach strips elicited by porcine pancreatic phospholipase A2 (PLA2). Contractions caused by arachidonic acid (AA), prostaglandin E2 (PGE2) and 5-hydroxytryptamine were not affected. No direct enzyme inhibition was observed in a radiochemical assay. 3. PGE2 release by both human fibroblasts and rat macrophages was reduced by peptide 204-212 in a dose-dependent manner. 4. The development of carrageenin-induced oedema in rats was significantly inhibited by the local administration of peptide 204-212. 5. The pattern and potency of the biological effects of peptide 204-212 are similar to those of antiflammin 2, a lipocortin 1-derived peptide. 6. It is suggested that peptide 204-212 may represent the active site responsible for the anti-inflammatory properties of lipocortin 5.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Annexin A5
  • Anti-Inflammatory Agents, Non-Steroidal*
  • Calcium-Binding Proteins / analysis
  • Calcium-Binding Proteins / pharmacology*
  • Carrageenan
  • Dinoprostone / metabolism
  • Edema / chemically induced
  • Edema / physiopathology
  • Humans
  • Male
  • Molecular Sequence Data
  • Muscle Contraction / drug effects
  • Muscle, Smooth / drug effects
  • Peptides / pharmacology*
  • Phospholipases A / antagonists & inhibitors
  • Phospholipases A2
  • Pregnancy Proteins / analysis
  • Pregnancy Proteins / pharmacology*
  • Rabbits
  • Rats
  • Rats, Inbred Strains
  • Recombinant Proteins / analysis
  • Recombinant Proteins / pharmacology
  • Sequence Alignment
  • Uteroglobin / analysis

Substances

  • Annexin A5
  • Anti-Inflammatory Agents, Non-Steroidal
  • Calcium-Binding Proteins
  • Peptides
  • Pregnancy Proteins
  • Recombinant Proteins
  • Carrageenan
  • Uteroglobin
  • Phospholipases A
  • Phospholipases A2
  • Dinoprostone