Bone marrow-derived progenitor T cells convey the origin of maturational arrest from CD4+CD8+ to CD4-CD8+ thymocytes in LEC mutant rats

Eur J Immunol. 1991 Sep;21(9):2277-80. doi: 10.1002/eji.1830210942.

Abstract

A mutant strain of rats, LEC, shows a novel arrest of T cell maturation from CD4+CD8+ to CD4+CD8- but not to CD4-CD8+ cells in the thymus. Transplantation of LEC rat fetal thymuses into the subcapsule of the kidney of athymic nude rats resulted in a normal maturation of thymocytes in the thymus graft. Furthermore, both single-positive thymocytes and peripheral lymph node T cells expressed T cell receptor alpha/beta antigen, and lymph node T cells acquired the ability to produce interleukin 2 upon mitogen stimulation. Transplantation of fetal thymuses from LEA rats, which express the same major histocompatibility complex haplotype as LEC rats, into LEC rat kidney subcapsule resulted in the maturational arrest from CD4+CD8+ to CD4+CD8- cells in the thymus graft. These data strongly suggest that bone marrow-derived progenitor T cells carry the cause of maturational arrest and that the thymic stroma of LEC rats has a normal potential to nurse thymocytes.

MeSH terms

  • Animals
  • Bone Marrow / physiology
  • Cell Differentiation / physiology
  • Flow Cytometry
  • Interleukin-2 / biosynthesis
  • Lymph Nodes / immunology
  • Rats
  • Rats, Inbred F344
  • Rats, Mutant Strains
  • Receptors, Antigen, T-Cell / biosynthesis
  • Receptors, Antigen, T-Cell, alpha-beta
  • T-Lymphocytes / cytology*
  • Thymus Gland / immunology*
  • Thymus Gland / transplantation

Substances

  • Interleukin-2
  • Receptors, Antigen, T-Cell
  • Receptors, Antigen, T-Cell, alpha-beta