Endomyocardial upregulation of beta1 adrenoreceptor gene expression and myocardial contractile reserve following cardiac resynchronization therapy

J Card Fail. 2008 Mar;14(2):172-8. doi: 10.1016/j.cardfail.2007.10.016.

Abstract

Background: Congestive heart failure (CHF) is associated with a blunted force-frequency relation (FFR) and myocardial contractile reserve (MCR) partially from a downregulation of beta1-adrenoreceptors (beta1-AR). We investigated whether acute and chronic cardiac resynchronization therapy (CRT) was capable of reversing the blunted FFR and MCR and if this was associated with upregulation of beta1-AR.

Methods and results: Left ventricle dP/dtmax was invasively measured in 10 CHF patients (New York Heart Association class > or =3; ejection fraction <25%) during incremental dual chamber (DDD)-CRT pacing at 70, 90, 110, and 130 beats/min, with and without continuous infusion of intravenous dobutamine, immediately after CRT implantation (BL) and 4 months later (FU). In a subgroup of 5 patients, serial left ventricle beta1 and beta2-AR gene expression was measured using reverse transcriptase-polymerase chain reaction. Four months after the initiation of resynchronization therapy, DDD-CRT pacing results in a significant upward shift of the heart rate versus LV dP/dtmax relationship (P < .01) with force frequency amplification as evidenced by the steeper slope of the force frequency response (P = .04). Infusion of dobutamine recruits myocardial contractile reserve and increases the heart rate versus LV dP/dtmax relationship at BL and at FU (both P < .05). However, only at follow-up was an additional force frequency amplification noticed (P < .05) during dobutamine infusion. This observation was paralleled by a significant upregulation of beta1-AR gene expression (P = .02).

Conclusions: Chronic CRT is associated with a partial restoration of the FFR and with a recruitment in myocardial contractile reserve, which is paralleled by upregulation of beta1-AR.

MeSH terms

  • Aged
  • Cardiac Output, Low
  • Cardiac Pacing, Artificial*
  • Dobutamine / pharmacology
  • Endocardium / physiology*
  • Exercise Tolerance
  • Female
  • Gene Expression
  • Heart Failure / genetics
  • Heart Failure / physiopathology*
  • Heart Rate
  • Humans
  • Male
  • Middle Aged
  • Muscle Contraction
  • Myocardial Contraction / genetics*
  • Myocardial Contraction / physiology
  • Myocardium*
  • Receptors, Adrenergic / genetics*
  • Receptors, Adrenergic / physiology
  • Stroke Volume
  • Ventricular Function, Left

Substances

  • Receptors, Adrenergic
  • Dobutamine