Screening of senescence-associated genes with specific DNA array reveals the role of IGFBP-3 in premature senescence of human diploid fibroblasts

Free Radic Biol Med. 2008 May 15;44(10):1817-32. doi: 10.1016/j.freeradbiomed.2008.02.001. Epub 2008 Feb 20.

Abstract

Repeated exposures to sublethal concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of WI-38 human diploid fibroblasts. We found 16 replicative senescence-related genes with similar alterations in expression level in replicative senescence and two models of stress-induced premature senescence. Among these genes was IGFBP-3. Using a siRNA approach, we showed that IGFBP-3 regulates the appearance of several biomarkers of senescence after repeated exposures of WI-38 fibroblasts to tert-butylhydroperoxide and ethanol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cellular Senescence*
  • Diploidy*
  • Ethanol / pharmacology
  • Fibroblasts / drug effects
  • Fibroblasts / physiology*
  • Gene Expression Profiling
  • Humans
  • Insulin-Like Growth Factor Binding Protein 3 / physiology*
  • Oligonucleotide Array Sequence Analysis
  • tert-Butylhydroperoxide / pharmacology

Substances

  • Insulin-Like Growth Factor Binding Protein 3
  • Ethanol
  • tert-Butylhydroperoxide