Seroepidemiology and molecular characterization of hepatitis E virus in Jilin, China

Infection. 2008 Mar;36(2):140-6. doi: 10.1007/s15010-007-7130-8. Epub 2008 Mar 12.

Abstract

Background: In order to develop region-specific diagnostic assays and prevent hepatitis E virus (HEV), it is essential to understand epidemiology and genotypic variation within different populations. However, the epidemiological data of HEV infections in Jilin is shortage.

Methods: The seroepidemiological study was conducted by enzyme-linked immunosorbent assay, and the conserved genomic sequences of open reading frame 2 (348 bp) was detected using reverse transcription-PCR.

Results: Overall, 1,289 serum samples were positive to HEV-specific immunoglobulin G (IgG), and 180 serum samples were positive to HEV-specific immunoglobulin M (IgM). The seroprevalence of HEV-specific IgG was 26.3%, 27.9%, 25.3%, 32.7% in 2003, 2004, 2005, 2006, respectively. Acute HEV infection mainly occurred in male, people aged from 20 to 69 were more susceptible to infection, and cases with IgM anti-HEV reaction mainly occurred from July to November. HEV RNA was detectable in the serum samples or stool suspension of 15 patients with HEV-specific IgM, and all of these belonged to genotype IV.

Conclusions: Our results indicate that HEV is widely spread in Jilin and confined to genotype IV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Chi-Square Distribution
  • Child
  • China / epidemiology
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Genotype
  • Hepatitis Antibodies / blood*
  • Hepatitis E / epidemiology*
  • Hepatitis E / immunology
  • Hepatitis E / virology
  • Hepatitis E virus / classification*
  • Hepatitis E virus / genetics
  • Hepatitis E virus / immunology*
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Male
  • Middle Aged
  • Phylogeny
  • Reverse Transcriptase Polymerase Chain Reaction
  • Seasons
  • Seroepidemiologic Studies*
  • Serotyping

Substances

  • Hepatitis Antibodies
  • Immunoglobulin G
  • Immunoglobulin M