Analysis of primary T cell responses to intact and fractionated microbial pathogens

K Pfeffer; B Schoel; H Gulle; H Moll; S Kromer; S H Kaufmann; H Wagner

doi:10.1007/978-3-642-76492-9_23

Analysis of primary T cell responses to intact and fractionated microbial pathogens

Curr Top Microbiol Immunol. 1991:173:173-8. doi: 10.1007/978-3-642-76492-9_23.

Authors

K Pfeffer¹, B Schoel, H Gulle, H Moll, S Kromer, S H Kaufmann, H Wagner

Affiliation

¹ Institute of Medical Microbiology and Hygiene, Technical University of Munich, FRG.

PMID: 1833139
DOI: 10.1007/978-3-642-76492-9_23

Abstract

Freshly isolated human T lymphocytes were tested for their response to mycobacteria, mycobacterial lysates, 2 dimensional (2D) PAGE separated mycobacterial lysates, leishmania and defined leishmanial antigen preparations. While gamma delta T cells proliferated vigorously in the presence of mycobacteria and mycobacteria derived lysates, a significant stimulation from 2 D gel separated lysates was not detected. In addition gamma delta T cells failed to respond towards leishmania or leishmanial components. In the alpha beta T cell compartment some donors, presumably according to their state of immunity against mycobacteria, responded to mycobacteria, mycobacterial lysates and 2 D gel separated mycobacterial lysates. Neither freshly isolated gamma delta T cells nor alpha beta T cells from naive donors did mount a significant immune response against leishmania.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Heat-Shock Proteins / immunology
Humans
In Vitro Techniques
Leishmania / immunology
Lymphocyte Activation
Mycobacterium / immunology
Receptors, Antigen, T-Cell, alpha-beta
Receptors, Antigen, T-Cell, gamma-delta
T-Lymphocyte Subsets / immunology*

Substances

Heat-Shock Proteins
Receptors, Antigen, T-Cell, alpha-beta
Receptors, Antigen, T-Cell, gamma-delta