Investigation of killer cell immunoglobulin-like receptor gene diversity in KIR3DL1 and KIR3DS1 in a transplant population

Tissue Antigens. 2008 May;71(5):434-9. doi: 10.1111/j.1399-0039.2008.01017.x. Epub 2008 Mar 10.

Abstract

Several overlapping amplicons were used to obtain the sequence of genomic DNA covering most of the coding regions of KIR3DL1 and KIR3DS1 from a family and 77 bone marrow transplant patients and their unrelated donors. Alleles 3DL1*00101 and *002 were most frequently observed in addition to 12 other known 3DL1 alleles. A single 3DS1 allele, 3DS1*01301, was identified in the 31 of 32 individuals carrying this gene. Two new alleles, 3DL1*01702 and 3DS1*058, were characterized. Three samples appeared to carry the duplicated killer cell immunoglobulin-like receptor (KIR) haplotype observed in other studies based on the presence of 3DS1 and two 3DL1 alleles. Additionally, one sample appeared to carry a novel KIR haplotype containing one 3DL1 and two 3DS1 alleles.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • B-Lymphocytes
  • Bone Marrow Transplantation*
  • Cell Line
  • Haplotypes
  • Humans
  • Killer Cells, Natural
  • Receptors, KIR3DL1 / genetics*
  • Receptors, KIR3DS1 / genetics*

Substances

  • Receptors, KIR3DL1
  • Receptors, KIR3DS1