Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species

J Clin Microbiol. 2008 May;46(5):1867-9. doi: 10.1128/JCM.00139-08. Epub 2008 Mar 12.

Abstract

The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). The clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Primers / genetics
  • Genes, rRNA*
  • Helicobacter / classification*
  • Helicobacter / genetics
  • Helicobacter / isolation & purification*
  • Helicobacter Infections / diagnosis*
  • Helicobacter Infections / microbiology
  • Humans
  • Polymerase Chain Reaction / methods*
  • RNA, Ribosomal, 16S / genetics*
  • Sensitivity and Specificity

Substances

  • DNA Primers
  • RNA, Ribosomal, 16S