Abstract
Secretion of the lysosomal enzyme hexosaminidase is induced by amphotericin B in mouse spleen adherent cells that show a significant increase in their candidacidal activity. The stimulation of beta-hexosaminidase is both time and dose dependent. Amphotericin B treatment did not change hexosaminidase expression that is represented mainly by "A-type" hexosaminidase in macrophages, on the basis of its biochemical properties.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylglucosamine / analogs & derivatives
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Acetylglucosamine / metabolism
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Amphotericin B / pharmacology*
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Animals
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Cells, Cultured
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Chromatography, DEAE-Cellulose
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Hymecromone / analogs & derivatives
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Hymecromone / metabolism
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Lysosomes / enzymology
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Macrophage Activation
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Macrophages / drug effects
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Macrophages / enzymology*
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Macrophages / immunology
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Mice
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Molecular Weight
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Spleen / chemistry
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Spleen / cytology
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Spleen / drug effects
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Spleen / enzymology
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Temperature
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beta-N-Acetylhexosaminidases / metabolism*
Substances
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4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside
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Hymecromone
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Amphotericin B
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beta-N-Acetylhexosaminidases
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Acetylglucosamine