Amelioration of collagen-induced arthritis by human recombinant soluble FcgammaRIIb

Clin Immunol. 2008 May;127(2):225-33. doi: 10.1016/j.clim.2008.02.002. Epub 2008 Mar 17.

Abstract

Immune complex (IC) binding to Fc gamma receptors (FcgammaRs) is central for inflammatory reactions seen in autoimmune diseases. Consequently, a therapeutic agent with a possibility to interfere with binding of pathogenic IC to FcgammaRs would be valuable in autoimmune disorders such as rheumatoid arthritis (RA). Here we have explored the therapeutic effect of a recombinant soluble human FcgammaRIIb (sFcgammaRIIb) protein in collagen-induced arthritis (CIA). In vitro studies of the sFcgammaRIIb demonstrated binding to mouse IgG, suggesting that sFcgammaRIIb can absorb pathogenic IgG anti-collagen type II (CII) IC in vivo. Hence, administration of sFcgammaRIIb significantly reduced CIA severity compared to control treated mice. The sFcgammaRIIb treated mice had significantly less IgG anti-CII antibodies in serum and lower mRNA levels of inflammatory cytokines compared to control mice. In conclusion, sFcgammaRIIb treatment ameliorates CIA by reducing IC-stimulated inflammation and joint swelling. This suggests that recombinant sFcgammaRIIb may be useful as therapeutic agent in RA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arthritis, Experimental / drug therapy*
  • Arthritis, Experimental / immunology*
  • Arthritis, Rheumatoid / drug therapy*
  • Arthritis, Rheumatoid / immunology*
  • Cytokines / genetics
  • Cytokines / immunology
  • Dose-Response Relationship, Immunologic
  • Enzyme-Linked Immunosorbent Assay
  • Foot / pathology
  • Histocytochemistry
  • Humans
  • Immunization
  • Immunoglobulin G / blood
  • Male
  • Mice
  • Mice, Inbred DBA
  • RNA, Messenger / chemistry
  • RNA, Messenger / genetics
  • Receptors, IgG / immunology*
  • Recombinant Proteins / pharmacology
  • Ribonucleases / chemistry

Substances

  • Cytokines
  • FCGR2B protein, human
  • Immunoglobulin G
  • RNA, Messenger
  • Receptors, IgG
  • Recombinant Proteins
  • Ribonucleases