The health of factory workers is a resource of business activity. However, at present, the rate of positive findings in the periodic medical checkups of factory workers is still increasing in Japan. After a 1-year health education program, we investigated the health awareness, a behavioral survey, and the data of the periodic medical checkups of factory workers. Most survey items of physical activities and mental health were significantly improved, and those of dietary habits and knowledge of lifestyle-related diseases also had a beneficial change. As an effective result, there was an improvement in most data of the physical examinations.