Abstract
A novel class II 5-enoylpyruvylshikimate-3-phosphate synthase (EPSPS) was identified from Pseudomonas stutzeri A1501 by complementation of an Escherichia coli auxotrophic aroA mutant. The single amino acid substitution of serine (Ser) for asparagine (Asn)-130 of the A1501 EPSPS enhanced resistance to 200 mM glyphosate. The mutated EPSPS had a 2.5-fold increase for IC(50) [glyphosate] value, a 2-fold increase for K (i) [glyphosate] value, but a K (m) [PEP] value similar to that of wild type. The effect of the single residue mutation on glyphosate resistance was also analyzed using a computer-based three-dimensional model.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3-Phosphoshikimate 1-Carboxyvinyltransferase / chemistry
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3-Phosphoshikimate 1-Carboxyvinyltransferase / genetics*
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Amino Acid Substitution
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Genes, Bacterial
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Glycine / analogs & derivatives*
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Glycine / pharmacology
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Glyphosate
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Herbicide Resistance / genetics*
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Herbicides / pharmacology*
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Kinetics
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Mutant Proteins / chemistry
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Mutation / genetics*
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Phylogeny
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Pseudomonas stutzeri / drug effects*
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Pseudomonas stutzeri / enzymology*
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Pseudomonas stutzeri / genetics
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Pseudomonas stutzeri / growth & development
Substances
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Herbicides
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Mutant Proteins
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3-Phosphoshikimate 1-Carboxyvinyltransferase
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Glycine