Baicalein stimulates osteoblast differentiation via coordinating activation of MAP kinases and transcription factors

Jin Mi Kim; Su-Ui Lee; Young Sup Kim; Yong Ki Min; Seong Hwan Kim

doi:10.1002/jcb.21760

Baicalein stimulates osteoblast differentiation via coordinating activation of MAP kinases and transcription factors

J Cell Biochem. 2008 Aug 1;104(5):1906-17. doi: 10.1002/jcb.21760.

Authors

Jin Mi Kim¹, Su-Ui Lee, Young Sup Kim, Yong Ki Min, Seong Hwan Kim

Affiliation

¹ Laboratory of Chemical Genomics, Center for Drug Discovery Technologies, Korea Research Institute of Chemical Technology, Daejeon, South Korea.

PMID: 18384125
DOI: 10.1002/jcb.21760

Abstract

The identification of anabolic agents that directly stimulate bone formation has recently attracted greater interest. Here, baicalein was identified as a natural compound that stimulates the differentiation of mouse osteoblastic MC3T3-E1 subclone 4 cells. Baicalein induced the activation of NF-kappaB in the initiation stage of osteoblast differentiation, and it activated the MAP kinase/NF-kappaB signaling pathway and induced the expression of osteoblast differentiation markers in the early stage. In the late stage, baicalein stimulated the calcium deposition with the activation of MAP kinases and AP-1 family members such as Fra-1 and Fra-2. Another transcription factor, NFATc1, was slightly induced by baicalein in the late stage. Thus, baicalein could stimulate the osteoblast differentiation via the activation of complexly coordinated signaling pathways that include MAP kinases and transcription factors such as NF-kappaB, AP-1, and NFATc1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkaline Phosphatase / metabolism
Animals
Biomarkers / metabolism
Calcification, Physiologic / drug effects
Cell Differentiation / drug effects*
Cell Differentiation / genetics
Cell Line
Cell Survival / drug effects
Enzyme Activation / drug effects
Flavanones / chemistry
Flavanones / pharmacology*
Gene Expression Regulation / drug effects
Mice
Mitogen-Activated Protein Kinases / metabolism*
NF-kappa B / metabolism
NFATC Transcription Factors / genetics
NFATC Transcription Factors / metabolism
Osteoblasts / cytology*
Osteoblasts / drug effects
Osteoblasts / enzymology*
Protein Transport / drug effects
RNA, Messenger / genetics
RNA, Messenger / metabolism
Transcription Factor AP-1 / genetics
Transcription Factor AP-1 / metabolism
Transcription Factors / metabolism*

Substances

Biomarkers
Flavanones
NF-kappa B
NFATC Transcription Factors
RNA, Messenger
Transcription Factor AP-1
Transcription Factors
baicalein
Mitogen-Activated Protein Kinases
Alkaline Phosphatase