Abstract
A highly emissive nucleobase analog, based on a thieno[3,4-d]pyrimidine core, is enzymatically incorporated into RNA oilgonucleotides that function as base discriminating fluorescent probes.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Base Pair Mismatch*
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DNA / chemistry
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry*
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Molecular Structure
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Nucleosides / chemical synthesis
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Nucleosides / chemistry*
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Oligonucleotides / chemistry*
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RNA / chemistry*
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Sensitivity and Specificity
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Time Factors
Substances
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7-ribofuranosylthieno(3,4-d)pyrimidine-3,6-dione
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Fluorescent Dyes
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Nucleosides
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Oligonucleotides
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RNA
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DNA