[Effect of prophages on transfer frequency of conjugative plasmid G 873]

V S Zueva; L N Nesterenko; O A Dmitrenko; W Witte

[Effect of prophages on transfer frequency of conjugative plasmid G 873]

Antibiot Khimioter. 1991 Oct;36(10):20-2.

[Article in Russian]

Authors

V S Zueva¹, L N Nesterenko, O A Dmitrenko, W Witte

Affiliation

¹ Institute of Experimental Epidemiology, Wernigerode, FRG.

PMID: 1839598

Abstract

Transfer of the conjugative plasmid G873 on filters and mixed cultivation of the donor and recipient cells in liquid media is described. In the both systems the use of the lysogenic recipient cells (phages of serogroups B and F) in the crossings increased mor than 100-fold the frequency of plasmid transfer. The conjugative transfer of the plasmid in the mixed cultivation system was proved. The conjugative transfer required the presence (while not obligatory) of calcium chloride and was restricted by the serum factors.

Publication types

English Abstract

MeSH terms

Animals
Calcium Chloride / pharmacology
Conjugation, Genetic / physiology*
Culture Media
Humans
In Vitro Techniques
Lysogeny / drug effects
Lysogeny / genetics
Methicillin / pharmacology*
Methicillin Resistance / genetics*
Plasmids / genetics
R Factors / drug effects
R Factors / genetics
Rabbits
Staphylococcus Phages / drug effects
Staphylococcus Phages / genetics*
Staphylococcus aureus / drug effects
Staphylococcus aureus / genetics*
Transduction, Genetic / drug effects
Transduction, Genetic / genetics*

Substances

Culture Media
Calcium Chloride
Methicillin