RNA editing of sorghum mitochondrial atp6 transcripts changes 15 amino acids and generates a carboxy-terminus identical to yeast

Curr Genet. 1991 Nov;20(5):417-22. doi: 10.1007/BF00317071.

Abstract

Sequencing of sorghum mitochondrial atp6 cDNA clones revealed 19 C-to-U transcript editing events within a 756 bp-conserved core gene; three were silent and 16 resulted in 15 amino acid changes. Only one edit, which was silent, was found in the 381 bp amino-extension to the core gene. Eleven of the 15 changed amino acids were identical with or else represented conservative changes compared to yeast atp6. Editing of a CAA codon to TAA truncates the carboxy-terminus to a position identical to that of yeast. The frequency of editing at sites which change amino acids was very high in contrast to partial editing at silent, third base, sites.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA, Mitochondrial
  • Edible Grain
  • Genetic Variation
  • Mitochondria / metabolism*
  • Molecular Sequence Data
  • Proton-Translocating ATPases / genetics*
  • Proton-Translocating ATPases / metabolism
  • RNA Processing, Post-Transcriptional*
  • Saccharomyces cerevisiae / genetics
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic*

Substances

  • DNA, Mitochondrial
  • Proton-Translocating ATPases