Plasma amyloid levels and the risk of AD in normal subjects in the Cardiovascular Health Study

Neurology. 2008 May 6;70(19):1664-71. doi: 10.1212/01.wnl.0000306696.82017.66. Epub 2008 Apr 9.

Abstract

Objectives: To examine the association between incident Alzheimer disease (AD), and plasma A beta 1-40 and A beta 1-42 levels in normal and mild cognitive impairment (MCI) subjects in a subgroup of participants of the Cardiovascular Health Study Cognition Study.

Methods: We determined the plasma A beta 1-40 and A beta 1-42 levels of 274 nondemented subjects (232 normals and 42 with MCI) in 1998-1999 and repeated the measurements in 2002-2003. The mean age of the subjects at baseline was 79.3 +/- 3.6 years. We examined the association between A beta levels and incident AD over the ensuing 4.5 years, controlling for age, cystatin C level (marker of glomerular function), apolipoprotein E-4 allele, Modified-Mini-Mental State Examination scores, and MRI-identified infarcts.

Results: In an unadjusted prospective model in normal subjects, both A beta 1-40 and A beta 1-42 levels in 1998-1999 were associated with incident AD (n = 55) in 2002-2003 (longitudinal analysis). In the fully adjusted multivariate model, neither A beta 1-42 nor A beta 1-40 nor their ratio was associated with incident AD. However, adjustment had a very small effect on point estimates for A beta 1-42, from an odds ratio (OR) of 1.61 (p = 0.007) in the unadjusted model to an OR of 1.46 (p = 0.08) in the fully adjusted model. In 2002-2003 (cross-sectional analysis), only the unadjusted models showed that both peptides were associated with AD.

Conclusions: Plasma A beta levels are affected by age and by systemic and CNS vascular risk factors. After controlling for these conditions, A beta-40 and A beta 1-42 are weak predictors of conversion to Alzheimer disease (AD) in normal subjects and are only weakly associated with AD in cross-sectional analysis. Consequently, plasma levels of A beta do not seem to be useful biomarkers for AD.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Age Factors
  • Aged
  • Aged, 80 and over
  • Alzheimer Disease / blood*
  • Alzheimer Disease / epidemiology*
  • Alzheimer Disease / physiopathology
  • Amyloid beta-Peptides / blood*
  • Apolipoprotein E4 / genetics
  • Biomarkers / analysis
  • Biomarkers / blood
  • Brain / metabolism*
  • Brain / pathology
  • Brain / physiopathology
  • Cerebrovascular Disorders / epidemiology
  • Comorbidity
  • Cross-Sectional Studies
  • Cystatin C
  • Cystatins / blood
  • Female
  • Humans
  • Incidence
  • Longitudinal Studies
  • Magnetic Resonance Imaging
  • Male
  • Models, Statistical
  • Neuropsychological Tests
  • Peptide Fragments / blood*
  • Predictive Value of Tests
  • Prospective Studies
  • Reference Values
  • Risk Factors

Substances

  • Amyloid beta-Peptides
  • Apolipoprotein E4
  • Biomarkers
  • CST3 protein, human
  • Cystatin C
  • Cystatins
  • Peptide Fragments
  • amyloid beta-protein (1-40)
  • amyloid beta-protein (1-42)