Folded structures of L-leucylglycine oligopeptides and their aggregational behavior in aqueous solution: Raman scattering spectra and proton NMR spin-lattice relaxation studies

J Phys Chem B. 2008 May 8;112(18):5824-33. doi: 10.1021/jp074732q. Epub 2008 Apr 12.

Abstract

The aggregational behavior of three L-leucylglycine oligopeptides (residue numbers of glycine are 3, 4, and 5) in aqueous solution was investigated by the use of Raman scattering and 1H NMR spin-lattice relaxation methods. The results indicate that their oligopeptides take up a folded structure to form dimeric aggregates above their critical aggregation concentration. The application of one-dimensional aggregate theory to these systems provides the following prediction. Elongation up to 6 glycine residues makes it possible to form dimeric aggregates, but further elongation (up to 7 glycine residues) makes the aggregates very unstable, and up to 8 or 9 glycine residues makes the formation of dimeric aggregates very difficult. The one-dimensional aggregate theory may be used to predict the existence of peptide aggregates through intermolecular hydrogen bonding.

MeSH terms

  • Dimerization
  • Dipeptides / chemistry*
  • Dipeptides / metabolism*
  • Hydrogen Bonding
  • Models, Molecular
  • Molecular Structure
  • Nuclear Magnetic Resonance, Biomolecular
  • Oligopeptides / chemistry*
  • Oligopeptides / metabolism*
  • Protein Folding*
  • Protons
  • Solutions
  • Spectrum Analysis, Raman

Substances

  • Dipeptides
  • Oligopeptides
  • Protons
  • Solutions
  • glycylleucine