Nanogap detector inside nanofluidic channel for fast real-time label-free DNA analysis

Nano Lett. 2008 May;8(5):1472-6. doi: 10.1021/nl080473k. Epub 2008 Apr 17.

Abstract

We report fabrication and characterization of a novel real-time, label-free DNA detector, that uses a long nanofluidic channel to stretch a DNA strand and a nanogap detector (with a gap as small as 9 nm) inside the channel to measure the electrical conduction perpendicular to the DNA backbone as it moves through the gap. We have observed electrical signals caused by 1.1 kilobase-pair (kbp) double-stranded (ds)-DNA passing through the gap in the nanogap detectors with a gap equal to or less than 13 nm.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Computer Systems
  • DNA / analysis
  • DNA / chemistry*
  • DNA / genetics*
  • Electrochemistry / instrumentation*
  • Electrochemistry / methods
  • Equipment Design
  • Equipment Failure Analysis
  • Microelectrodes
  • Microfluidics / instrumentation*
  • Microfluidics / methods
  • Nanotechnology / instrumentation*
  • Nanotechnology / methods
  • Oligonucleotide Array Sequence Analysis / instrumentation*
  • Oligonucleotide Array Sequence Analysis / methods
  • Sequence Analysis, DNA / instrumentation*
  • Sequence Analysis, DNA / methods
  • Staining and Labeling

Substances

  • DNA