Pathological aspects of spontaneous uveitis and retinopathy in HLA-A29 transgenic mice and in animal models of retinal autoimmunity: relevance to human pathologies

Ophthalmic Res. 2008;40(3-4):175-80. doi: 10.1159/000119872. Epub 2008 Apr 18.

Abstract

Purpose: A major increased risk of developing birdshot chorioretinopathy is reported in humans who are HLA-A29-positive. To better characterize this disease, an animal model of HLA-A29-associated disease was developed and the pathology arising spontaneously in these transgenic mice was compared to animal models of autoimmune uveoretinitis and to human pathology.

Materials and methods: HLA-A2902 cDNA (A29c) was obtained from a patient suffering from birdshot retinochoroidopathy and used for transgene construct to generate HLA-A29 transgenic mice. Histopathological examination of the animal cohort was performed up to 15 months of age. It was compared with the ocular pathology developed in C57BL/6 mice and in Lewis rats immunized with retinal autoantigens.

Results: Aging HLA-A29 transgenic mice spontaneously developed an ocular disease with resemblance to experimental retinal-Ag-induced autoimmune ocular disease and to human pathologies shown in birdshot retinochoroidopathy, Vogt-Koyanagi-Harada and sympathetic ophthalmia. Pathogenic mechanisms could possibly be shared by these conditions.

Conclusion: Humanized models of ocular inflammation developed in HLA class I and class II transgenic mice will help better understand the mechanisms responsible for ocular inflammation. Local control of autoimmunity in HLA-A29-positive individuals would be an important option for new therapeutic strategies.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Autoimmune Diseases / immunology
  • Autoimmune Diseases / pathology*
  • Autoimmunity / immunology*
  • Disease Models, Animal*
  • Disease Progression
  • Female
  • Follow-Up Studies
  • HLA-A Antigens / immunology
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Rats
  • Rats, Inbred Lew
  • Retina / immunology*
  • Severity of Illness Index
  • Uveitis / immunology
  • Uveitis / pathology*
  • beta 2-Microglobulin / immunology

Substances

  • HLA-A Antigens
  • HLA-A29 antigen
  • beta 2-Microglobulin