Abstract
Virulent variants of Rice yellow mottle virus (genus Sobemovirus) can emerge on the highly resistant rice cultivars Gigante and Bekarosaka. Non-synonymous mutations responsible for the breakdown of the recessive resistance gene rymv1-2 were located in the VPg after determination of its termini in the polyprotein P2a. The secondary structure of this protein was predicted to include a central alpha-helix. The two major amino acids related to virulence are located on the same side of this helix. The 3D topology and the biochemical properties of virulence mutations both suggested a direct site-to-site interaction between RYMV VPg and rice eIF(iso)4G encoded by rymv1.
MeSH terms
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Amino Acid Sequence
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Eukaryotic Initiation Factor-4G / chemistry
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Eukaryotic Initiation Factor-4G / metabolism*
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Models, Molecular
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Molecular Sequence Data
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Oryza
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Plant Diseases / immunology
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Plant Diseases / virology
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Plant Proteins / chemistry
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Plant Proteins / metabolism*
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Plant Viruses / chemistry*
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Plant Viruses / pathogenicity
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Protein Binding
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Protein Structure, Tertiary / genetics*
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RNA Viruses / chemistry*
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RNA Viruses / pathogenicity
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Ribonucleoproteins / genetics*
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Ribonucleoproteins / metabolism*
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Viral Nonstructural Proteins / genetics*
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Viral Nonstructural Proteins / metabolism*
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Virulence
Substances
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Eukaryotic Initiation Factor-4G
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Plant Proteins
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Ribonucleoproteins
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Viral Nonstructural Proteins